Determination of Eight Lignans in Schisandrae Sphenantherae Fructus on the Market from Different Habitats by UPLC-MS/MS
OBJECTIVE To compare the contents of schizandrin A,schizandrin B,schizandrin C,schisandrol A,schisandrol B,schisantherin A,anwuligan,and schisanhenol in Schisandrae Sphenantherae Fructus on market from 12 habitats.METHODS The samples were pre-treated by 96-well fitration plates.The assay was performed on ACE EXCEL 1.7 C18-AMIDE(100 mm×2.1 mm,1.7 μm)column with 0.1％formic acid-water(A)and methanol(B),gradient elution,flow speed was 0.4 mL·min-1.Ion source was electric spray ion source,positive ion monitoring mode,multireaction monitoring mode for quantitative analysis.Linear,instrument precision,stability,repeatability,average recovery were investigated.RESULTS The content of schisantherin A in 10 of 12 producing areas reached the standard of ≥0.2％of Schisandrae Sphenantherae Fructus in 2020 Edition of Chinese Pharmacopoeia.CONCLUSION UHPLC-MS/MS is suitable for simultaneous determination of multiple components in Schisandrae Sphenantherae Fructus.The Schisandrae Sphenantherae Fructus in the market basically meet the national legal standards.
万方数据
维普
