Effect of LncRNA OIP5-AS1 on Phenotypic Transformation of IOSE80 in Ovarian Epithelium
OBJECTIVE To explore the phenotypic changes and possible mechanisms of lncRNA OIP5-AS1 on the proliferation,migration,apoptosis,invasion and cycle of ovarian epithelial cells IOSE80.METHODS The clinical data of patients were collected from TCGA database and GEO database.After R package analysis,the differential expression of OIP5-AS1 was visualized in the volcanic map.The correlation between survival rate and OIP5-AS1 was analyzed by Kaplan-Meier.The IOSE80 cell model of OIP5-AS1 over expression and silencing was constructed with lentivirus vector.The expression of OIP5-AS1 was verified by RT-qPCR.Cell proliferation was detected by CCK-8.Invasion was detected by Transwell.Cell migration was detected by scratch test.Cell cycle and apoptosis were detected by flow cytometry.Western blotting was used to detect the expression of E-cadherin and N-cadherin,as well as the expression of cyclin-dependent kinase(CDK)and cyclin-G-related kinase(GAK).RESULTS RT-qPCR results showed that IOSE80 cell lines over expressing and silencing OIP5-AS1 were successfully constructed.CCK-8 results showed that overexpressing OIP5-AS1 promoted the proliferation of IOSE80 cells.Scratch test results showed that overexpressing OIP5-ASl promoted the migration of IOSE80 cells.Transwell results showed that overexpressing OIP5-AS1 would increase the invasiveness of IOSE80 cells.Flow cytometry results showed that overexpression of OIP5-AS1 weakened the apoptosis of IOSE80 cells and promoted the progress of cell cycle.Western blotting results showed that overexpression of OIP5-AS1 downregulated the expression of E-cadherin and upregulated the expression of N-cadherin,while overexpression of OIP5-AS1 increased the expression of CDK and GAK proteins.CONCLUSION LncRNA OIP5-ASl further interferes with the regulation of IOSE80 cell cycle by up regulating the expression of CDK and GAK,and then indirectIy regulates the malignant phenotype of ovarian epithelial cells.
万方数据
维普
