Study on the Rule of Berberine Hydrochloride Through Carboxymethyl Chitosan-sodium Alginate Polyelectrolyte Hydrogel
OBJECTIVE To investigate the transfer and diffusion of berberine hydrochloride(BBH),the main active component of Coptis and Phellodenticum in the system of carboxymethyl chitosan(CMCS)-sodium alginate(SA).METHODS CMCS and SA were stirred in a certain proportion,and D-gluconolactone(GDL)was added to form polyelectrolyte hydrogel.Rheometer was used to study the rheological properties of CMCS-SA hydrogel,including the elastic modulus G'and the viscous modulus G".A BBH diffusion model for CMCS-SA hydrogel was designed,and the relevant rules of BBH permeation through CMCS-SA polyelectrolyte hydrogel were observed by UV-VIS.RESULTS The elastic modulus G'of the hydrogel was measured when the ratio of CMCS to SA was 3∶1,2∶1,1∶1,1∶2,and 1∶3.When the ratio of CMCS to SA was 1∶1,G'was the highest,and the crosslinking strength of the hydrogel was the highest.The cumulative amount of BBH transfer was measured by the BBH transfer model,and the diffusion of BBH in CMCS-SA hydrogel was fitted as the skeleton dissolution by Peppas equation,indicating that BBH dissociation and the transfer efficiency increased as the amino group of CMCS decreased or the carboxyl group of SA increased.The elastic modulus G'of CMCS-SA hydrogel increased with the increase of GDL content.The reason was that the binding force between CMCS and SA molecules gradually increased with the decrease of pH,and the crosslinking degree of the hydrogel was enhanced.When ratio of fixed CMCS to SA was 1∶1 and the GDL content was 0.15 g·mL-1,the formability of CMCS-SA hydrogel was good.In addition,when BBH was transferred in hydrogel with different concentrations of GDL,the transfer efficiency increased with the increase of GDL content.When BBH was delivered in different thickness hydrogel,the delivery efficiency of CMCS-SA hydrogel increased with the decrease of thickness.CONCLUSION The CMCS-SA hydrogel system,as a potential drug carrier for traditional Chinese medicine extracts such as BBH,is expected to serve as a gel carrier for transdermal drug delivery.
万方数据
维普
