A Rapid,Hyperspectral-based Method for Determining Sporoderm-broken Rate of Ganoderma Lucidum Spore Powder
OBJECTIVE To establish a rapid nondestructive detection method for the sporoderm-broken rate of Ganoderma lucidum spore powder by hyperspectral technology combined with chemometrics.METHODS Hyperspectral images of Ganoderma lucidum spore powder samples with different sporoderm-broken rates were collected,and spectral data in the visible-shortwave near-infrared band(397-1 004 nm)range of each sample were calculated after selecting the region of interest.Compared 6 spectral preprocessing methods[standard normal variable transformation,multivariate scattering correction,Savitsky-Golay(SG)smoothing,wavelet transform,SG smoothing+standard normal variable transformation,and SG smoothing+multivariate scattering correction],5 characteristic band extraction methods(competitive adaptive reweighting,successive projections algorithm,uninformative variables elimination,least angle regression,and genetic algorithm),and 5 algorithms(partial least squares regression,support vector regression,extreme leaming machine,multilayer perceptron,and LightGBM)for constructing quantitative correction models to predicts performance.RESULTS The optimal combination was SG smoothing+competitive adaptive reweighted feature band selection+partial least squares.The quantitative correction model established based on the algorithm combination achieved a prediction set coefficient of 0.868 2,and a root mean square error of 0.011 7 for Ganoderma lucidum spore powder samples with a sporoderm-broken rate range of 90％-100％.The selected optimal algorithm combination was applied to construct a quantitative correction model with a sporoderm-broken rate range of 0-100％,the coefficient of determination for the test set was 0.973 1 and the root mean square error was 0.049 3,showing good generalization ability.CONCLUSION The established quantitative detection model can realize the rapid and non-destructive detection of the sporoderm-broken rate of Ganoderma lucidum spore powder,which provides technical support for the quality control of Ganoderma lucidum spore powder and its products.
hyperspectral imagingGanoderma lucidum spore powdersporoderm-broken ratechemometricsquantitative calibration model
万方数据
维普
