Simultaneous Determination of Nine Bile Acid Components in Shedan Chuanbei Liquid by UHPLC-MS
OBJECTIVE To establish an UHPLC-MS method for simultaneous determination of 9 bile acids components in Shedan Chuanbei liquid.To analyze the differences of bile acids components from different manufacturers and different batches of product.METHODS Thermo Fisher Scientfic Bremen HYPERSIL GOLD(2.1 mm×100 mm,1.9 μm)was selected as the chromatographic column.The mobile phase was 0.1％formic acid-water and methanol with gradient elution at a flow rate of 0.2 mL·min-1,and the column temperature was 40 ℃.HESI negative ion mode was used for mass spectrometry,and PRM scanning mode was used for mass spectrometry data.RESULTS The calibration curves of 9 biles acids were linear in their own range(r≥0.999 2).The average recoveries ranged from(95.5±1.52)％to(103.1±3.81)％.The RSD ranged from 1.1％to 5.5％.Nine bile acids components including taurocholic acid,tauroursodesoxycholic acid,7-ketone-3α,12-α-hydroxyl cholanic acid,taurochenodeoxy-cholic acid,sodium taurodeoxycholate,cholic acid,sodium taurocholate,chenodeoxycholic acid and deoxycholic acid were tentatively identified from 19 batches of test samples.Taurocholic acid,taurochenodeoxycholic acid and taurodeoxycholate sodium were detected in all the test batches.CONCLUSION This method is simple and stable,and has certain reference value for improving the quality control and evaluation of Shedan Chuanbei liquid.
万方数据
维普
