OBJECTIVE To study the efficacy evaluation of triptolide(TP)in rats with cerebral ischemia-reperfusion(I/R)injury and its mechanism.METHODS Rat brain I/R injury model was copied by middle cerebral artery wire embolism surgery,and TP(0.1,0.2 mg·kg-1)was given to the treatment group,and set the sham surgery group.The Longa score method was used to measure the neural function of rats,and Niselferi staining was used to show the morphology of neurons in the ischemic side brain tissue of rats,immunofluorescence was used to detect the expression levels of MAP2 and Syn in ischemic lateral brain tissue.The expression levels of cAMP,PKA,BDNF,Syn and PSD-95 were detected by Western blotting.RESULTS Compared with the model group,the neurological scores of TP treatment group decreased significantly(P＜0.01 or P＜0.001),it had a protective effect on damaged neurons.Compared with the model group,cAMP,PKA,BDNF,Syn and PSD-95 in TP treatment group were significantly up-regulated.CONCLUSION TP treatment can significantly improve I/R injury,and the mechanism may be related to the activation of the cAMP/PKA/BDNF signaling pathway.
维普
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