Simultaneous Determination of 14 Components in Qingfei Yihuo Tablets by HPLC
OBJECTIVE To establish a high-performance liquid chromatography method for the simultaneous determination of 14 components including geniposide,mangiferin,baicalin,berberine hydrochloride,wogonoside,baicalein,aloe-emodin,rhein,wogonin,emodin,praeruptorin A,chrysophanol,physcion and praeruptorin B in Qingfei Yihuo tablets.METHODS Titank C18(250 mm×4.6 mm,5 μm)column was used;0.1％phosphoric acid solution(A)-acetonitrile(B)was used as the mobile phase with gradient elution;detection wavelengths:geniposide at 238 nm,aloe-emodin,rhein,emodin,chrysophanol,physcion and mangiferin at 254 nm,berberine hydrochloride at 265 nm,baicalin,wogonoside,baicalein,and wogonin at 280 nm,praeruptorin A and praeruptorin B at 321 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 35 ℃;the injection volume was 10 μL.RESULTS The linear ranges of geniposide,mangiferin,baicalin,berberine hydrochloride,wogonoside,baicalein,aloe-emodin,rhein,wogonin,emodin,praeruptorin A,chrysophanol,physcion and,praeruptorin B were 4.96-223.17,0.84-42.22,18.76-938.16,4.46-223.17,4.86-243.10,1.59-79.32,0.76-38.17,1.03-51.49,1.59-79.40,1.21-60.72,1.80-90.06,0.91-45.48,1.04-51.83 and 0.86-43.23 μg·mL-1,with r all≥0.999 9.The RSDs of instrument precision,stability and reproducibility tests were＜3％,and the average recoveries in sample(n=6)were＞90％,with the RSDs＜3％.CONCLUSION The method is simple and reproducible and can provide a scientific basis for improving the quality standard of Qingfei Yihuo tablets.
Qingfei Yihuo tabletsHPLC-DADcontent determinationquality control
万方数据
维普
