Effect of Irradiation Sterilization on the Quality of Rubus Chingii Hu
OBJECTIVE To study the effect of 60Co-γ irradiation on the sterilization effect and main components of Rubus chingii Hu.METHODS Irradiated Rubus chingii Hu by 0,6,10,15,30 kGy doses of 60Co-γ,used the microbial count method to determine the microbial level of Rubus chingii Hu before and after irradiation.Analyzed the components of Rubus chingii Hu by high resolution mass spectrometry,investigated the effects of irradiation on the quality of Rubus chingii Hu by comparing the components of Rubus chingii Hu samples before and after irradiation,analyzing the quantitative results of ellagic acid and kaempferol 3-O-yunxiangoside,and evaluating the similarity of fingerprints.RESULTS The results of microbial examination of Rubus chingii Hu after different doses of irradiation all met the requirements,cluster analysis and principal component analysis of 20 components showed no significant difference.And there was no significant difference in the contents of ellagic acid and kaempferol 3-O-glucoside before and after irradiation.The similarity of fingerprints before and after irradiation was between 0.995 and 1.000.CONCLUSION Irradiation can effectively control the microbial level in Rubus chingii Hu,and there is no significant effect on the chemical composition of Rubus chingii Hu,the results provide a basis for the application of irradiation in the sterilization process of Rubus chingii Hu.
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