Vildagliptin Protects Palmitic Acid-induced Myocardial Inflammatory Damage in Diabetes Mellitus
OBJECTIVE To explore the mechanism of vildagliptin,a dipeptidyl peptidase 4 inhibitor(DPP-4i),in improving the inflammatory damage of cardiomyocytes induced by diabetes mellitus.METHODS The AC 16 cardiomyocytes cultured in vitro were randomly divided into blank group,palmitic acid(PA)group,0.1,1,10 μmol·L-1 vildagliptin group,and sitagliptin group(positive control group).The cell viability was detected by CCK-8 kit.The expressions of DPP-4,p-NF-κB and IκB were detected by Western blotting.The expression level of inflammatory cytokines TNF-α and IL-6 were detected by ELISA kit.TUNEL kit was used to detect cell apoptosis.RESULTS After PA treatment,the cell morphology of AC 16 human cardiomyocytes changed,CCK-8 results showed a decrease in cell survival rate,Western blotting results showed increased phosphorylation of NF-κB and increased expression of DPP-4 protein,and ELISA results showed increased expression level of inflammatory factors TNF-α and IL-6.The increase of TUNEL positive ratio promoted apoptosis of cardiomyocytes.After administration of DPP-4i vildagliptin,it could effectively inhibit the abnormal expression of DPP-4 protein induced by PA,improve the morphology of cardiomyocytes,and down-regulate the level of NF-κB phosphorylation.ELISA results showed that vildagliptin could improve the expression level of PA-induced inflammatory factors TNF-α and IL-6,and reduce the proportion of TUNEL positive(P＜0.05).CONCLUSION Vildagliptin can effectively antagonize PA-induced inflammatory injury of cardiomyocytes,antagonize myocardial injury induced by diabetic cardiomyopathy by inhibiting the phosphorylation level of NF-κB,reducing the expression level of intracellular inflammatory factors and inhibiting apoptosis.
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