GC-MS Analysis of Total Volatile Oil from Branches and Leaves of Four Rhododendron Species Under Supercritical CO2 Extraction
OBJECTIVE To clarify the components of volatile oil in branches and leaves of Rhododendron anthopogonoides,Rhododendron capitatum,Rhododendron thymifolium and Rhododendron przewalskii.METHODS The total volatile oil in leaves and branches of these plants were obtained by supercritical CO2 extraction.After that,the chemical composition of the total volatile oil was analyzed and identified by GC-MS,and the contents of different parts and varieties were compared.RESULTS The results showed that the highest oil yield of leaves was Rhododendron thymifolium(6.97％),and the highest oil yield of branches was Rhododendron anthopogonoides(20.53％).Thirty-five,eighty,fifty-eight and forty compounds were detected in the branch oil of Rhododendron anthopogonoides,Rhododendron capitatum,Rhododendron thymifolium and Rhododendron przewalskii respectively,among which Rhododendron capitatum was rich in compounds.Forty-eight,fifty-seven,sixty-two and fifty compounds were detected in the leaf oil,among which the compounds of Rhododendron anthopogonoides were the richest.Among the detected components,squalene(34.92％,26.90％)was the highest content in the branches and leaves of Rhododendron anthopogonoides.1-Eicosanol(26.79％)was the highest content in the branch oil of Rhododendron capitatum,and octadecyl acetate(42.32％)was the highest content in the leaf oil.The highest content of bisabola-3,10-diene-2-one(34.66％,28.20％)was found in the branches and leaves of Rhododendron thymifolium,and 15-oxoETE(38.20％,40.40％)was the highest content in the branches and leaves of Rhododendron przewalskii.The results showed that the contents of oil in branches and leaves of Rhododendron capitatum were quite different in different parts.In the comparison of different varieties,the compounds with the highest content of four rhododendrons were all different.CONCLUSION According to the difference of the content of active components of different rhododendrons and parts,the appropriate species and parts for purposeful development and utilization should be selected.The research results can provide scientific basis for rational development and utilization of Rhododendron resources.
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