Simultaneous Determination of 2 Specific Flavonoids Glycosides in Dendrobium Officinale by UHPLC-Q-TOF-MS
OBJECTIVE To establish an UHPLC-Q-TOF-MS method for the simultaneous determination of apigenin-6-C-(2"-O-β-D-glucopyranosyl)-α-L-arabino pyranoside and apigenin-8-C-(2"-O-β-D-glucopyranosyl)-α-L-arabinopyranoside in Dendrobium officinale.METHODS The separation was performed on an Agilent ZORBAX SB C18(100 mm×2.1 mm,1.8 μm)column with a mobile phase consisting of acetonitrile(A)and 0.1％formic acid water solution(B)with gradient elution at a flow rate of 0.25 mL·min-1,the column temperature was 30 ℃ and the injection volume was 2 μL.The MS spectrometry detection was performed with electrospray ionization in negative mode,the scanning rang was m/z 110-1 700.RESULTS Apigenin-6-C-(2"-O-β-D-glucopyranosyl)-α-L-arabinopyranoside and apigenin-8-C-(2"-O-β-D-glucopyranosyl)-α-L-arabinopyranoside were showed good linear relationships within the ranges of 0.004 96-0.248 μg·mL-1(r=0.999 9)and 0.010 79-0.537 9 μg·mL-1(r=0.999 9).The average recoveries were 99.04％and 100.41％,RSD were 2.09％and 2.00％,respectively.CONCLUSION This method is simple,rapid,sensitive,with good specificity,and it can provide a reference for the quality control of Dendrobium ofiicinale.
Dendrobium officinaleUHPLC-Q-TOF-MScontent determinationquality control
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