基于多指标-响应面法优化土炒白术的炮制工艺
Process Optimization of Soil-fried Atractylodis Macrocephalae Rhizoma Based on the Multi-Index-Response Surface Method
袁楚 1刘想 1高如汐 1王凡一 1张可 1吴昊 1刘思奇 1赵启苗 2单国顺1
作者信息
- 1. 辽宁中医药大学,辽宁大连 116600
- 2. 辽宁医药职业学院,沈阳 110101
- 折叠
摘要
目的 对土炒白术的炮制工艺进行优化.方法 采用紫外可见分光光度法和超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS)技术对土炒白术中多糖含量以及白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ等活性成分的含量进行测定,采用色差仪对土炒白术粉末色度值进行分析;将上述化学成分的含量以及饮片的色度作为评价指标,采用单因素试验法对土炒白术过程中的辅料用量、炒制温度、炒制时间进行考察;通过层次分析-赋值(AHP-CRITIC)混合加权法结合Box-Behnken响应面法进行土炒白术炮制工艺的优化研究.结果 优化土炒白术的最佳炮制工艺为每50 g药材用灶心土28.4 g,在204℃下炒制5.30 min.结论 本实验确定的土炒白术工艺切实可行,可为该炮制品的工业化生产提供一定的指导意义.
Abstract
OBJECTIVE To optimize the processing technology of soil stir-fried Atractylodis Macrocephalae Rhizoma(AMR).METHODS UV-visible spectrophotometry and ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS)was used to determine the content of polysaccharides and active ingredients such as atractylenolide Ⅰ,atractylenolide Ⅱ,atractylenolide Ⅲ,etc.The colorimeter was used to determine the color value of soil-fried AMR powder.The contents of the above chemical components and the color value of soil-fried AMR were used as evaluation indexes.The single factor trial method was adopted to investigate the amount of excipients,stir-frying temperature and time of AMR.The analytic hierarchy process and criteria importance through inter criteria correlation(AHP-CRITIC)combined with the method of Box-Behnken response surface was used to design and optimize the processing technology of soil stir-fried AMR.RESULTS The best processing technology of soil fried AMR were optimized to 28.4 g of soil added to 50 g of medicinal materials which was fried 5.30 min in 204 ℃.CONCLUSION The process of soil stir-fried AMR determined in this experiment is feasible which can provide certain guiding significance for the industrial production of the processed products.
关键词
白术/土炒/混合加权法/Box-Behnken响应面法/工艺优化Key words
Atractylodis Macrocephalae Rhizoma/soil-fried/mixed weighting method/Box-Behnken response surface method/process optimization引用本文复制引用
基金项目
国家自然科学基金青年基金项目(81803726)
辽宁省教育厅面上项目(LJKMZ20222205)
辽宁省教育厅面上项目(LJKMZ20221308)
辽宁中医药大学校级课题重点项目(2021LZY054)
辽宁医药职业学院校级课题(BSKY202405)
出版年
2024
NETL
NSTL
万方数据