Research on Effect of Huangjin Capsule on Prevention and Treatment of Rats with Non-alcoholic Fatty Liver Disease by Ferroptosis
OBJECTIVE To investigate the effect of Huangjin capsule on inflammation and liver function of non-alcoholic fatty liver disease(NAFLD)rats through ferroptosis.METHODS Forty-eight SPF male SD rats were randomly divided into blank control group,model group,positive control group,high-dose,medium-dose and low-dose of Huangjin capsule group.Blank control group was fed ordinary diet,and the other groups were fed high-fat diet to prepare NAFLD rat model.After 8 weeks of modeling,the high-dose,medium-dose and low-dose of Huangjin capsule groups were given different doses of Huangjin capsule suspension by intragastric administration,the blank control group and the model group were given equal volume of normal saline intragastric administration,and the positive control group was given deferoxamine(0.1 g·kg-1)intraperitoneal injection for continuous 4 weeks.The pathological changes of liver were observed by HE staining after 4 weeks.Tumor necrosis factor α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8)and interleukin-1β(IL-1β)of serum were determined by ELISA.Total cholesterol(TC),triglycerides(TG),alanine transferase(ALT)and aspartate transaminase(AST)levels of serum were detected by automatic biochemical analyzer.The morphology and protein expression of rat liver were observed by immunohistochemistry.The protein and mRNA expressions of ferritin heavy chain(FTH1),nuclear factor E2 related factor 2(Nrf2),Kelch-like epichlorohydrin-associated protein 1(Keap1),heme oxygenase-1(HO-1)and quinone oxidoreductase-1(NQO1)were detected by Western blotting and RT-qPCR.RESULTS HE results showed that in the model group,the structure of hepatocyte cord was damaged,bleeding,a little fibrosis,fat vacuoles were more,partial necrosis and dissolution of hepatocytes,and a large number of inflammatory cells were infiltrated.In the positive control group,there was only a small amount of hepatocyte necrosis and inflammatory cells.Steatosis,inflammatory cell infiltration and hepatocyte damage were also significantly reduced in the high-dose and medium-dose of Huangjin capsule groups,while there were still more inflammatory cells in the low-dose group,hepatocyte damage and bleeding were still obvious but steatosis was less than in the model group.Compared with normal control group,the contents of IL-8,IL-6,TNF-α and IL-1β in serum and the levels of TC,TG,ALT and AST in model group were significantly increased,while the expressions of FTH1,HO-1,Nrf2,Keapl and NQO1 in liver tissue were significantly decreased(P＜0.01).Compared with model group,the contents of IL-8,IL-6,TNF-α and IL-1β and the levels of TC,TG,ALT and AST of Huangjin capsule groups and positive control group were significantly decreased,while the expressions of FTH1,HO-1,Nrf2,Keapl and NQO1 were significantly increased(P＜0.05 or P＜0.01).The changes of high dose group and positive control group were more significant(P＜0.01).CONCLUSION Huangjin capsule may alleviate inflammation by inhibiting ferroptosis,reduce TC and TG levels in serum,improve liver function and liver pathological damage of NAFLD,and has potential therapeutic effect on NAFLD.
万方数据
维普
