Study on the Effect and Mechanism of Yiwei Decoction in the Treatment of N-methyl-N'-nitro-N-nitrosoguanidine-induced Chronic Atrophic Gastritis Rats and Gastric Cancer Rats
OBJECTIVE To explore the effect and potential molecular mechanisms of Yiwei Decoction on chronic atrophic gastritis(CAG)and gastric cancer.METHODS N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)was used to induce the model of CAG/gastric precancerous lesions and gastric cancer in rats.Therapeutic effect of Yiwei Decoction after intervention was observed.High-throughput sequencing technology and RT-qPCR were used to detect the change of transcriptome in stomach and blood of MNNG-induced tumor rats.RESULTS Yiwei Decoction significantly ameliorated the MNNG-induced CAG and intestinal metaplasia in rats,and significantly reduced the weight of tumor at gastroduodenal junction.Induction of MNNG obviously increased the mRNA level of Vcan,BGIG10116_32332 and rno-miR-542-3p in rat stomach and rno-miR-363-3p in rat serum,while Yiwei Decoction reversed these change.Moreover,Yiwei Decoction could slightly reduce the expression of gastric cancer therapeutic targets including VEGFR and HER-2.CONCLUSION Yiwei Decoction is effective in the treatment of MNNG-induced CAG and intestinal metaplasia;and it can further inhibit the progression of MNNG induced gastric and duodenal tumors in rats.Its pharmacological mechanism may be achieved by inhibiting the overexpression of Vcan,lncRNA BGIG10116-32332,and mo-miR-542-3p in the stomach,as well as suppressing the overexpression of mo-miR-363-3p in the blood.
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