OBJECTIVE To prepare Citri Sarcodactylis Fructus and Caryophylli Flos(Citri Sarcodactylis Fructus∶Caryophylli Flos)=(1∶3)essential oil liposome gel and investigate its anti-Propionibacterium acnes(P.acnes)mechanism.METHODS Thin film hydration-high pressure homogenization method was used to prepare Citri Sarcodactylis Fructus and Caryophylli Flosessential oil liposomes,then further loaded on carbomer matrix to obtain Citri Sarcodactylis Fructus and Caryophylli Flos essential oil liposomes gel.Fourier transform infrared spectroscopy and differentia scanning calorimetry was used to analysis the loading state of essential oil;screen index components of essential oil by gas chromatography-mass spectrometry;modified Franz diffusion cell method was used to study the transdermal penetration and dermal retention of Citri Sarcodactylis Fructus and Caryophylli Flos essential oil liposomes gel.Inhibition mechanism of Citri Sarcodactylis Fructus and Caryophylli Flos essential oil liposomes gel was investigated by detecting the inhibition rate and content of alkaline phosphatase,biofilm formation and total protein.RESULTS Citri Sarcodactylis Fructus and Caryophylli Flos essential oil was loaded in the hydrophobic zone of the lipid carrier.D-Limonene and eugenol were the index components of Citri Sarcodactylis Fructus and Caryophylli Flos essential oil.Citri Sarcodactylis Fructus and Caryophylli Flos essential oil liposomes gel could improve the stability and skin retention of essential oils.Citri Sarcodactylis Fructus and Caryophylli Flos essential oil liposomes gel inhibited P.acnes up to(65.9±1.7)％at essential oil level of 1.068 mg·mL-1.It could inhibit bacterial growth by destroying bacterial cell wall and membrane structure and reducing bacterial protein synthesis.CONCLUSION Citri Sarcodactylis Fructus and Caryophylli Flos essential oil liposomes gel helps to improve the stability of essential oils and exerts a slow-release effect,prolongs the antibacterial effect of the drug,and achieves long-term antibacterial effect.
维普
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