Rapid Analysis of Chemical Components in Massa Medicata Fermentata Based on UHPLC-LTQ-Orbitrap-MS/MS Technology
OBJECTIVE To establish a rapid and efficient ultra high performance liquid chromatography-linear ion trap orbital trap tandem mass spectrometry(UHPLC-LTQ-Orbitrap-MS/MS)method for systematic and comprehensive qualitative analysis of chemical components in Massa Medicata Fermentata.METHODS UHPLC-LTQ-Orbitrap-MS/MS was used to scan the precise molecular weight of the compounds in Massa Medicata Fermentata,and accurate qualitative analysis of Massa Medicata Fermentata was achieved through reference substance and multi-stage fragment ion information,combined with relevant literature and fragmentation rules.RESULTS A total of 76 chemical components were identified,including amino acids,nucleosides,flavonoids,organic acids,phenylpropanoids,terpenoids,cyanosides,unsaturated fatty acids,etc.,and the peak area of 76 components before and after fermentation of Massa Medicata Fermentata was compared,and a total of 8 components with significant changes in peak area were found.CONCLUSION A rapid qualitative analysis method for the chemical components of Massa Medicata Fermentata is established.This study provides a theoretical basis for the quality evaluation index and the pharmacodynamic material basis of Massa Medicata Fermentata.
Massa Medicata FermentataUHPLC-LTQ-Orbitrap-MS/MSamino acidsnucleosidesflavonoidsphenylpropanoidsterpenoidscyanosides
万方数据
维普
