Simultaneous Determination of 11 Components in Niaodukang Mixture by UHPLC-ESI-MS/MS
OBJECTIVE To established a UHPLC-ESI-MS/MS method for simultaneous determination of gallic acid,calycosin-7-glucoside,rutin,hyperoside,salvianolic acid B,rhein,astragaloside Ⅳ,cryptotanshinone,emodin,tanshinone Ⅰ,tanshinone ⅡA in Niaodukang mixture.METHODS InertSustainR C18(3.0 mm× 100 mm,3.0 μm)column was used,mobile phase was methanol(A)-0.l％formic acid(B)with gradient elution at a flow rate of 0.4 mL·min-1,and the injection volume was 20 μL.The mass spectrometry used the electrospray ionization source(ESI source).Multiple-reaction monitoring was employed with switching electrospray ion source polarity in positive and negative mode.RESULTS The peak area and mass concentration of 11 components in Niaodukang mixture showed a good linear relationship within the linear range.The precision of RSD were＜3.0％,the average recoveries ranged from 98.44％to 103.56％,RSD ranged from 2.04％to 3.34％.CONCLUSION The method is stable,accurate,and simple,which can be used to control and evaluate the quality of Niaodukang mixture.It can also provide evidence for clinical application and research of Niaodukang mixture.
万方数据
维普
