Performance and Application of Monoclonal Antibodies Analysis of Domestic Chip Capillary Electrophoresis Instrument
OBJECTIVE To compare the performance parameters of the chip capillary electrophoresis instrument independently developed in China base on MCE-SDS with the PA800 Plus capillary electrophoresis instrument,and the application of reducing monoclonal antibody sample detection with domestic capillary electrophoresis.METHODS A systematic examination of the domestic chip capillary electrophoresis apparatus in terms of linearity,sensitivity and precision using the mixture of human serum albumin and transferrin.Meanwhile,the application of domestic capillary electrophoresis instrumentation for the detection of reduced monoclonal antibody protein samples was further investigated,along with methodological validation.RESULTS The results showed that this domestic chip capillary electrophoresis instrument had good system performance and could complete the detection and analysis of protein samples quickly and in high throughput.CONCLUSION This domestic chip capillary electrophoresis instrument has the ability of protein analysis and detection,and can be used for the purity analysis of general protein samples and monoclonal antibody drugs.
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