Preparation of the Baicalein-Zinc Complex Nano-delivery System and Effect on Inflammatory Bowel Disease in Mice
OBJECTIVE To prepare Baicalein-Zinc(Ba-Zn)complex nano-delivery system for the treatment of inflammatory bowel disease(IBD)in mice.METHODS The Ba-Zn complex was prepared,and it was modified with chondroitin sulfate(CS)to self-assembly prepare Ba-Zn@CS nano-delivery system.Ba-Zn@CS nanoparticles were characterized by Fourier transform infrared spectrometer(FT-IR)and transmission electron microscope(TEM),and its particle size,potential were determined.The therapeutic effect of the Ba-Zn@CS nano-delivery system was evaluated in the IBD mice from aspects such as the disease activity index(DAI),the length of the colon length,and the pathological analysis of colon tissue pathology.RESULTS FT-IR and TEM proved that the successful construction of Ba-Zn@CS nanoparticles with a particle size of 356.4 nm,polydispersity index(PDI)of 0.129,surface zeta potential of-35.7 mV.The in vivo efficacy evaluation showed that the weight loss of the Ba-Zn@CS treatment group was significantly alleviated,the DAI was reduced,and the colon length was longer than the mice of the model group(P＜0.05).The pathological examination of colon tissue showed that the mice in the Ba-Zn@CS treatment group had a significant alleviating local inflammation of the colon.CONCLUSION The Ba-Zn@CS nano-drug delivery system improve the in vivo anti-IBD effect of free Ba drugs,alleviate local inflammation in IBD mice,and provide a new approach to enhance the bioavailability of Ba for the treatment of IBD.
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