Identification of Chemical Constituents and Blood-absorbed Ingredients of Chaihu-Jia-Longgu-Muli Decoction by UPLC-Q-TOF-MSE
OBJECTIVE To rapidly identify the chemical constituents and blood-absorbed ingredients of Chaihu-Jia-Longgu-Muli decoction and analyze its cracking rule by UPLC-Q-TOF-MSE technology combined with UNIFI software.METHODS ChromCore C18(3.0 mm× 100 mm,3 μm)column was used for gradient elution with acetonitrile(A)-0.1％formic acid aqueous solution(B)as the mobile phase,the flow rate was 0.3 mL·min-1,and the sample size was 10 μL.Mass spectrometry was used to detect the water substance and drug-containing plasma of Chaihu-Jia-Longgu-Muli decoction in positive and negative ion modes,respectively.UNIFI software was used to automatically search and match compounds according to relative molecular weight,characteristic fragment ion,retention time and other information,and qualitative identification of compounds was performed in combination with standards and literature.RESULTS One hundred and two compounds were identified in this study,including 38 saponins,32 flavonoids,12 polyphenols,9 anthraquinones and 11 Other compounds.A total of 21 prototype consituent were identified from rat plasma samples,10 from Ginseng Radix Et Rhizoma,5 from Scutellaria Baicalensis,2 from Bupleuri Radix,2 from Rhei Radix et Rhizoma,2 from Pinellia Rhizoma and l from Zingiberis Rhizoma Recens.CONCLUSION The application of UPLC-Q-TOF-MSE technology combined with UNIFI software can quickly and accurately analyze the chemical constituents and blood-absorbed ingredient of Chaihu-Jia-Longgu-Muli decoction,and initially clarify the pharmacodynamic material basis of Chaihu-Jia-Longgu-Muli decoction,which provides a basis for subsequent pharmacodynamic research and quality control of Chaihu-Jia-Longgu-Muli decoction.
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