Isolation,Purification and Structural Characterization of New Isomer in Posaconazole
OBJECTIVE To isolation,purify and identify the chemical structure of new isomer in posaconazole.METHODS The novel isomer of posaconazole was isolated and purified by semi-preparative HPLC,and the column was Luna C18(2)(250 mm× 20 mm,10 μm),and the mixture of acetonitrile-water containing 0.1％formic acid was used as mobile phase and the pure new isomer was successfully obtained.The isomer was quickly identified by LC-MS and NMR techniques.RESULTS The novel isomer was identified as 4-(4-(4-(4-(((3R,5R)-5-((1H-1,2,4-triazol-1-yl)methyl)-5-(2,4-difluorophenyl)tetrahydrofuran-3-yl)methoxy)phenyl)piperazin-1-yl)phenyl)-1-((2S,3S)-2-hydroxypentan-2-yl)-1H-1,2,4-triazol-5(4H)-one,and it was identified for the first time.CONCLUSION The separation and structural characterization of posaconazole isomer is of great significance to the quality control of posaconazole.
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