首页|一氧化氮提高黄瓜幼苗耐涝性的探究

一氧化氮提高黄瓜幼苗耐涝性的探究

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为了提高黄瓜幼苗的耐涝性,以100μmol·L-1硝普钠(SNP)作为一氧化氮供体,研究了一氧化氮对淹水胁迫下黄瓜幼苗细胞质膜、光合特性和抗氧化酶活性的影响.结果表明,外源一氧化氮能抑制淹水胁迫期间黄瓜根质膜相对透性和丙二醛含量的增加,淹水8d时,经SNP处理的黄瓜根质膜相对透性和丙二醛含量与对照分别达到显著和极显著差异;能延缓叶片叶绿素含量的下降,使淹水期间叶片能维持较高的光合能力,淹水8d时,经SNP处理的黄瓜叶片叶绿素含量和光合速率极显著高于对照;外源一氧化氮能提高淹水胁迫时黄瓜根SOD、POD、CAT的活性,淹水8d时,经SNP处理的黄瓜根SOD、POD活性与对照达到极显著差异,CAT活性与对照达到显著差异.研究表明外源一氧化氮能提高黄瓜幼苗的耐涝性.
Enhancement of waterlogging tolerance of cucumber seedlings by nitric oxide
In order to enhance the waterlogging tolerance of cucumber seedlings,100 μmol· L-1 sodium nitroprusside (SNP) was used as a donor of exogenous nitric oxide (NO),the effects of exogenous NO on the plasma membrane,photosynthetic characteristics and antioxidant enzyme activities of cucumber seedlings under waterlogging stress were explored.The results indicated that exogenous NO could inhibited the increase of relative plasmalemma permeability and malondialdehyde of cucumber seedlings under waterlogging stress.The relative plasma membrane permeability and MDA content of the cucumber root treated by SNP were extremely significant different from that of control under waterlogging stress for 8 days.Exogenous NO could retard the decomposing of chlorophyll,maintain the photosynthetic capacity of cucumber seedlings under waterlogging.The chlorophyll content and photosynthetic rate of cucumber leaves treated by SNP were extremely higher than that of control group under waterlogging stress for 8 days.Exogenous NO could increase the activities of SOD,POD,CAT.The activities of SOD and POD of the cucumber root treated by SNP were highly significant and that of CAT were significant different from that of control group under waterlogging stress for 8 days.This experiment showed that exogenous NO could impove the capacity of anti-waterlogging stress of cucumber seedlings.

CucumberNitric oxideWaterlogging tolerance

张健、刘美艳

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江苏师范大学生命科学学院整合植物生物研究所 江苏徐州221116

黄瓜 一氧化氮 耐涝性

转基因生物新品种重大专项转基因生物分子特征识别技术江苏高校优势学科建设工程项目:2014年徐州市科技计划项目

2011ZX08012-002XF13C056

2017

中国瓜菜
中国农业科学院郑州果树研究所

中国瓜菜

北大核心
影响因子:0.452
ISSN:1673-2871
年,卷(期):2017.30(1)
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