The mutagenesis study was carried out with the bottle gourd seeds of ‘Ansheng’ in order to construct gourd mutant population.To screen the optimal condition of ethyl methanesulfonate (EMS),we analyzed the effect of the method of treatment for seed coat,the concentration and treatment time of EMS on seed germination rate.It was found the sensitivity to EMS was improved significantly when seed coat was opened.Therefore,under the condition of opening coat,we set up six different concentrations of EMS (0.8％,1％,1.5％,2％,3％ and 4.5％) and four different induced times (2,4,8 and 12 h) to analyze the seed relative germination in 24 different combinations of concentration and time.The results showed that the relative germination rate was reduced when the inducing time increased at the same concentration.But exceeding over 8 h,the effect was significant.In the same time,the relative germination rate was reduced with increasing EMS concentration.In this study,the optimum treatment with EMS mutagenesis was that the coat opened gourd seeds are soaked in warm water for 4 h,then treated with 1.5％ of EMS for 8 h,the median lethal concentration was 1％-2％.
NETL
NSTL
维普
万方数据
