Molecular screen for gf gene in six brown tomato varieties
There are five mutant genes including gf,gf2,gf3,gf4,gf5 for GF allele.The mutant gene result in red tomato turn into green and brown tomato.The brown tomatoes is one of the most favored fruits by people for its special fruit colour.Our latest research showed that gf mutant gene was strongly resistant to Tomato Chlorosis Virus (ToCV).In order to establish rapid molecular screen method for gf gene,6 brown tomato varities were chosen from 300 candidates as materials and the red tomato ‘2015-47’ was used as control.The result showed that none of 109 bp and 57 bp specific bands were digested for varity ‘T2016-33’ that was gf mutant;a187 bp and a 171 bp specific bands were amplified for varity ‘2012-2’ that was gf 2mutan;a 216 bp and a 18 bp specific bands were amplified for‘T2015-66’ and ‘T2016-32’that were gf4 mutant.In this experiment we established a rapid and exact method to identify the gf mutant gene that accelerate brown tomato breeding.
NETL
NSTL
万方数据
维普
