The genome and infectious clone of zucchini yellow mosaic virus melon isolate
Viral infectious clone is a powerful tool for studying the interaction between virus and host.In this study,the genome sequence of zucchini yellow mosaic virus(ZYMV)melon isolate CH-87 was cloned and analyzed,and its full-length cDNA clone was constructed.The results showed that the genome of isolate CH-87 was 9592 nt in length,and the average nucleotide and amino acid sequence identities between CH-87 and other isolates were 91.42％and 96.45％,respectivly.Phylogenetic analysis based on CP and polyprotein amino acid sequences showed that CH-87 was most closely related to isolate BL-67 from The United States,followed by isolate CN∶Lc∶17 from China,and they all clustered in subgroup V.The inoculation showed that the infectious clone was successfully constructed,and it could systematically infect melon,cucumber,watermelon and zucchini.The progeny produced from the clone was infectious by mechanical inoculation.
NETL
NSTL
万方数据
维普
