Optimisation of haploid induction techniques in watermelon with differ-ent fruit types
Unfertilized ovary culture of watermelon is an effective method to induce haploids,which can shorten the breeding time.In order to establish an efficient and stable haploid induction system and improve the haploid induction rate,the unfertilized ovaries of six watermelon hybrids(2n=2x=22)of large-fruited variety Xuelong 1 and Xuelong 3,medium-fruited variety Zaomi,Hongdu 2,and small-fruited variety Xiaoyu 9,Huangxiaoyu were used as experimental materials.The effects of different plant growth regulators on the haploid induction rate of watermelon were studied.The results showed that six different genotypes of watermelon were induced in the induction medium 2 mg·L-16-BA+1 mg·L-1 NAA,2 mg·L-1 6-BA+2 mg·L-1 NAA,and haploid regeneration plants were obtained except for Huangxiaoyu.Among them,Hongdu 2 had the highest induction rate of 63.89％,followed by Zaomi with an induction rate of 55.56％.
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NSTL
万方数据
维普
