Comparative study on chromosome doubling effect of watermelon hap-loid with different inducers and induction methods
Asbtract:In order to investigate the effect of different inducers and induction methods on chromosome doubling of water-melon haploid,the haploid plants obtained from watermelon anther culture in vitro were used as materials.Different con-centrations of colchicine and fluralin were used to study the effect of inducing diploid plants from haploid plants by three methods:medium doubling method,germination doubling method and droplet growing point doubling method.The results showed that the induction rate of haploid doubling by different doubling methods varied.The survival rate of plants induced by colchicine doubling reached 100％,and the mortality rate of plants induced by fluralin doubling reached 100％.When induced by tissue culture,the doubling induction rates of colchicine and trifluralin were the highest at a mass concentration of 60 mg·L-1,with 50.00％and 41.67％,respectively.When inducing doubling by soaking the buds with 600 mg·L-1 colchicine for 4 hours,the highest doubling induction rate was 75.00％;the highest doubling induc-tion rate of 300 mg·L-1 fluralin soaking buds for 2 hours was 50.00％.When inducing doubling at the droplet growth point,the highest doubling rate induced by 600 mg·L-1 colchicine was 50.00％;the highest induction doubling rate of 500 mg·L-1 trifluralin was 27.78％.Therefore,the effect of inducing haploid watermelon plants to double with colchicine is better than that with trifluralin,and the soaking method has the best effect on inducing doubling.
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NSTL
万方数据
维普
