大白菜蜡质缺失突变体YW71的遗传及序列变异分析
Inheritance and sequence variation analysis of a wax-less mutant YW71 in Chinese cabbage(Brassica rapa L.ssp.pekinensis)
杨双娟 1唐昊 2赵艳艳 1魏小春 1王志勇 1苏贺楠 1张文静 1李林 1王坐京 1原玉香 1张晓伟1
作者信息
- 1. 河南省农业科学院蔬菜研究所 郑州 450002
- 2. 河南省农业科学院蔬菜研究所 郑州 450002;河南科技学院园艺园林学院 河南新乡 453003
- 折叠
摘要
以大白菜蜡质突变体YW71为对象,研究其亮绿无蜡粉性状的遗传规律和调控基因.通过遗传分析,表明YW71中的亮绿无蜡粉性状由隐性单基因控制.通过等位基因互补实验证明YW71的亮绿性状是BrWAX2等位突变引起的.序列分析表明,在YW71中BrWAX2基因在第3个外显子末端发生了 39 bp的缺失,进而引起转录水平的可变剪切和翻译水平的提前终止.表达模式分析表明,BrWAX2基因在YW71茎和叶中表达量显著下降.此外,研究针对39 bp的变异开发并验证了共显性标记BrWAX2-InDe11.研究结果丰富了白菜类蔬菜蜡质突变遗传资源,将为亮绿无蜡粉品种的分子育种提供理论指导和技术支持.
Abstract
In this study,we identified a wax-less Chinese cabbage(Brassica rapa)mutant YW71,the inheritance and the controlling gene for the glossy trait were studied extensively.Genetic analysis indicated that the glossy trait in YW71 was controlled by a single recessive locus.Allelic complementary experiment showed that the glossy trait in YW71 was caused by mutation of the gene BrWAX2.Sequence analysis revealed that a 39 bp deletion was identified at the end of the third exon of BrWAX2,resulting in alternative splicing at the transcription level and finally leading to a premature stop codon at the translation level.Expression analysis showed that BrWAX2 was significantly down-regulated in stems and leaves of glossy YW71.Furthermore,a co-dominant marker BrWAX2-InDel was developed and validated.Overall,these results enrich the genetic resources of glossy mutants and provide applicable markers for marker-assisted selection(MAS)-based breeding of Chinese cabbage,which has pivotal significance in theory and practice.
关键词
大白菜/亮绿突变体/等位基因互补实验/序列变异分析Key words
Chinese cabbage/Glossy mutant/Allelic complementary experiment/Sequence variation analysis引用本文复制引用
基金项目
河南省农科院优秀青年科技基金(2022YQ10)
河南省自然科学基金(222300420197)
国家自然科学基金(32202485)
河南省农科院科技创新团队项目(2022TD06)
河南省重点研发专项(221111110100)
河南省农业良种联合攻关项目(2022010504)
现代农业产业技术体系建设专项(CARS-23-G15)
出版年
2024
NETL
NSTL
维普
万方数据