Effect of exogenous glutathione on the synthesis of glucosinolates in broccoli
To investigate the effects of glutathione(GSH)on glucosinolate(GSLs)biosynthesis,the effects of different concentrations of reduced glutathione(GSH),oxidized glutathione(glutathione disulfide;GSSG),and glutathione inhibitor(BSO)were studied on the content of glucosinolate and related substrates,enzyme activity,and gene expression in broccoli floret,using the broccoli variety Naihanyouxiu at the budding stage as experimental material.The results showed that,compared to CK,the 5 mg·L-1 GSH treatment significantly increased the total GSL and aliphatic GSL content in broccoli floret at 48 h.It significantly increased cysteine(Cys)content at 24-48 h,significantly increased glutathione content at 6-24 h,and significantly increased the expression of genes related to GSLs biosynthesis at 3-12 h.The 45 mg·L-1 GSH treatment significantly reduced the total GSL and aliphatic GSL content in the broccoli floret at 48 h,while significantly increasing cysteine content at 3-48 h and glutathione content at 6-48 h,and significantly inhibiting the expression of genes related to GSLs biosynthesis at 3-12 h.When compared with CK,the treatment with 5 mg·L-1 GSSG resulted in a significant increase in the content of total GSL,aliphatic GSL in broccoli floret,while treatments with 25,45,and 65 mg·L-1 GSSG had no effect on the content of total GSL,aliphatic GSL.In conclusion,exogenous glutathione has a concentration effect on the GSLs content,with low concentrations promoting GSLs biosynthesis while high concentrations inhibiting GSLs biosynthesis.
万方数据
维普
