Biological control effects of Bacillus velezensis SM2 against Botrytis cine-rea causing tomato gray mold
The aim was to find an excellent strain for efficient biological control and biocontrol mechanism of tomato gray mold(Botrytis cinerea).Endophytic bacteria SM2 of tomato root was isolated and purified by plat marking.The inhibitory effect of the strain SM2 on mycelial growth of B.cinereawas studied in dual cultures on PDA plates.Furthermore,the strain SM2 was identified according to physiology and biochemical characteristics and 16S rDNA sequencing.The biocontrol effect of the strain SM2 against B.cinerea was determined by pot and field experiments,and the physio-bio-chemical characteristics of tomato leaves in biocontrol group,disease group and control were analyzed.The results showed that strain SM2 was identified as Bacillus velezensis,which could cause mycelium distortion of B.cinerea,with inhibition rate of 66.67%.Pot and field investigation showed that B.velezensis SM2 effectively reduced the incidence and disease index of B.cinerea,and the biocontrol efficiency reached 71.73% and 65.22%,respectively.Compared with the control,SOD activity,APX activity,proline content and soluble protein content of tomato leaves in the biocontrol group and disease group were significantly increased,and the increase rate of biocontrol group was significantly higher than that of disease group.The above results demonstrate that B.velezensis SM2 is a valuable biocontrol strain,which can effectively suppress tomato gray mold through micro-antagonism and induce the increase of antioxidant enzymes activity and osmo-regulation substances content of tomato.
TomatoBotrytis cinereaBacillus velezensisAntagonismInduce systemic resistanceBiological control
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