Effects of NaCl stress on the germination of Brassica campestris seeds and seedling growth
By analyzing the effects of NaCl stress on seed germination and seedling growth of Brassica campestris,provide a basis for screening salt tolerant germplasm resources and cultivating excellent varieties.Huainan Tadiwu No.3 was used as the experimental material to conduct seed germination and seedling growth stress tests at concentrations of 0,0.05,0.1,0.2,0.3,0.4 mol·L-1,and the phenotypic,physiological and biochemical indicators related to seed germination and seedlings were measured.The results showed that with the increase of NaCl concentration,the germination potential,germination percentage,radicle and plumule length of Brassica campestris seeds decreased significantly.Compared with the seedling stage of Brassica campestris,its germination stage was more sensitive to salt stress;At the seedling stage,with the increase of NaCl stress concentration,the root fresh weight,root dry weight,above ground mass,and leaf width of Brassica campestris exhibited low concentration promoting growth and high concentration inhibiting growth.The leaf length,plant height,and leaf width of the seedlings were significantly lower than the control and gradually decreased with the increase of stress concentration.The relative chlorophyll content showed a trend of first significant increase and then decrease,and the MDA content and proline content were significantly higher than the control.The POD activity was significantly higher than the control and showed a significant increase and then gradually decreased.In conclusion,the concentration of 0.05 mol·L-1 NaCl significantly inhibited the germination rate and potential of Brassica campestris,but had a significant promoting effect on the plumule length of Brassica campestris embryos.When the concentration of NaCl solution was higher than 0.2 mol·L-1,above ground fresh mass,dry mass,and plant height of seedlings was signifi-cantly inhibited.MDA and Pro content,peroxidase activity showed a significant increase and then decrease trend,which can be used as an indicator to evaluate the salt tolerance of Brassica campestris under NaCl stress.
Brassica campestrisSalt stressSeed germinationPhysiological and biochemical indexes
万方数据
维普
