Optimization of the genetic transformation system in watermelon
In this study,eight watermelon genotypes were used as experimental materials,the germination rates and adventitious buds induction rates of different genotypes were counted and analyzed to screen out the optimal genotype with strong regeneration ability.The efficient watermelon genetic transformation system was optimized and screened through different sowing times,explant injury methods,bacterial liquid concentration,co-culture time,infection methods and other factors.The results showed that the seed germination rates of YL,CYY298,CYY171 and CYY137 had no dif-ference and were significantly higher than that of other materials at 2 days of seeding,but the germination induction rate of YL was the highest at different concentrations of GA3.The system was optimized based on YL material,and it was found that the seed germination state was the best and most suitable for infection after 2 days of sowing.The fluorescence brightness of the explants after scratching the blade was the strongest,and the fluorescence rate was up to 74.9%.When the concentration of bacterial solution was OD600=0.8 and the co-culture time was 3 days,the explants were in the best differentiation state and the fluorescence rate was the highest at 70.3%.The infection efficiency can be significantly improved by negative pressure infection,and the fluorescence rate of explants can reach 71.8%,and the callus induction rate can reach 60.2%.The induction of adventitious shoots was up to 74%after the addition of 0.5 mg·L-1 GA3 to the differentiation medium.The highest rooting rate and the highest average number of roots were observed when 0.5 mg·L-1 of IBA was added to the rooting medium.
万方数据
维普
