The metabolome and transcriptome jointly resolve the response mecha-nism of flavonoids in broccoli sprouts to exogenous ABA
In order to investigate the mechanism of action of the phytohormone abscisic acid on the synthesis of flavo-noids in broccoli sprouts,the cauliflower Naihan Youxiu was used as the test material.The metabolome and transcriptome were jointly analyzed in treated broccoli sprouts with exogenous spraying of 50 μmol·L-1 ABA while using water as control.The results showed that a total of 14 categories of substances were detected in the metabolome,including 212 flavonoids.Among them,there were 30 kinds of flavonoids with significant differences.The content of catechin gallate,epicatechin gallate and morin hydrate increased significantly,while those of naringin,argyragin and lycioside decreased significantly.A total of 13 regulatory genes related to flavonoid synthesis pathway,phenylpropane synthesis pathway and ABA signaling pathway were screened by combining metabolome and transcriptome analysis.Among them,LOC106337270,LOC106329559 and LOC106326133 showed significant positive correlation with the content of catechin gallate,epicatechin gallate and morin hydrate.Therefore,we speculated that these genes could be involved in regulating the biosynthesis of flavonoids in response to ABA signals.In summary,this study characterized the key genes of broccoli sprouts in response to exogenous ABA and regulation of flavonoid synthesis,which lay a scientific theoretical foundation for the subsequent regulation of flavonoid biosynthesis.
BroccoliFlavonoidsTranscriptomeMetabolomeExogenous ABA
万方数据
维普
