Study on seed vigor and physio-biochemical characteristics of fresh-Cu-curbita moschata seeds of different maturity
To explore the changes of seed vigor and physio-biochemical characteristics of fresh-Cucurbita moschata seeds during the maturation period,a self-breeding variety was used as materials to analyze the relationship of the vigor and physio-biochemical indexes of seeds with different maturity.The results showed that the seeds gradually developed plump morphology with the maturity,and the seed coat was obviously lignified.After 45 days post-anthesis(dpa),the seeds began to appear germinating ability and reached a peak at 70 dpa.During 35-50 dpa,the accumulation of stored substances was basically completed,and large quantities of fat,protein and starch were formed,while the content of total soluble sug-ar and soluble reducing sugar continued to decrease.With increased seed maturity,the activities of SOD,CAT and POD in seeds after 8 h imbibition were affected by MDA content,with a trend of first increasing and then decreased.The content of endogenous hormones varied,with of SA and BR showing a significant increasing trend overall and higher than other hormones,while GA3 and ABA showed an opposite trend,and ETH was the highest at 35 dpa.Correlation analysis indicated that seed vigor indexes were positively correlated with SOD activity,POD activity,the contents of starch,crude fat,CTK,GA3,IAA,BR and SA,but negatively correlated with the contents of total soluble sugar,soluble reducing sugar,protein,MDA,ABA and ETH.In conclusion,the development of fresh-Cucurbita moschata seeds was accompanied by the accu-mulation of storage substances,the increase and decrease of antioxidant enzyme activities and MDA content,the change of endogenous hormone content and the complex physio-biochemical process.
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