Research progress on in vitro gynogenesis technology of Cucurbitaceae crops
Haploid or double haploid plays an important role in crop improvement and genetic breeding.It is very important for increasing the induction frequency of haploid and establishing an efficient and stable in vitro gynogenetic induction system.The conventional breeding cycle is long,the workload is heavy,and the genetic characters are unstable.Haploid or double haploid plants are usually produced by in vitro culture of unpollinated ovary and ovule,so as to obtain homozy-gous inbred lines,which can make genes homozygous quickly and shorten breeding life greatly.In this paper,the authors reviewed the techniques of in vitro gynogenesis of cucurbit crops,and found that the main factors affecting the results,including the genotypes of the donor plants,the medium and added exogenous hormones,the methods of dark heat shock and pre-cooling,the sowing season,and the stage of ovary development.Besides,the methods of ploidy and doubling of regenerated plants were also summarized,to provide reference for the subsequent research on the development of in vitro female nuclei of cucurbitaceae crops.
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