摘要
MYB转录因子是植物转录因子家族中数量最多、用途最广的成员之一,为挖掘更多番茄(Solanum lycopersi-cum)MYB转录因子家族成员信息,初步探究其表达模式及功能,以番茄AilsaCraig为试材,采用RT-PCR的方法克隆SlMYB48基因,并对其进行生物信息学及表达、定位分析.结果表明,番茄SlMYB48基因的开放阅读框(ORF)长度为708 bp,编码235个氨基酸,在番茄的根中表达量最高,叶中次之.S1MYB48蛋白含有保守的MYB结构域,定位于细胞核中,属于不稳定、亲水性蛋白.对SlMYB48启动子分析,发现其含有大量的逆境响应元件,qRT-PCR及RNA-seq数据库分析结果表明,高盐、生物逆境胁迫条件下,SlMYB48基因表达量均随处理时间延长而升高,干旱胁迫条件下表达量下降,推测其可能参与番茄生物及非生物逆境胁迫反应.研究结果为进一步深入探究番茄SlMYB48基因的功能及作用机制提供了参考.
Abstract
MYB transcription factor is one of the most abundant and widely used members of the plant transcription fac-tor family.In order to mine more information about MYB transcription factor family members of tomato(Solanum lycop-ersicum)and preliminarily explore its expression pattern and function,this study took tomato Ailsa Craig as the test mate-rial.SlMYB48 gene was cloned by RT-PCR,and its bioinformatics,expression and localization were analyzed.The results showed that the open reading frame(ORF)length of tomato SlMYB48 gene was 708 bp,encoding 235 amino acids,and the expression level was the highest in tomato roots,followed by in leaves.The S1MYB48 protein contains a conserved MYB domain,which is an unstable,hydrophilic protein located in the nucleus.SlMYB48 promoter analysis found that it contained a large number of stress response elements.The results of qRT-PCR and RNA-seq database analysis showed that the expression of SlMYB48 gene increased with the extension of treatment time under high salt and biological stress,and decreased under drought stress,suggesting that SlMYB48 gene might be involved in biological and abiotic stress response of tomato.The results of this study provide a reference for further investigation of the function and mechanism of tomato SlMYB48 gene.
基金项目
烟台市科技计划(2022XCZX091)
现代农业产业技术体系建设专项(CARS-23-G11)
重庆市巫山县科技计划(wskjdxbxm2023004)
国家自然科学基金面上项目(32372737)
西南作物基因资源发掘与利用国家重点实验室开放基金(SKL-KF202224)
NETL
NSTL
万方数据