Bioinformatics and expression analysis of tomato SlMYB48 gene
MYB transcription factor is one of the most abundant and widely used members of the plant transcription fac-tor family.In order to mine more information about MYB transcription factor family members of tomato(Solanum lycop-ersicum)and preliminarily explore its expression pattern and function,this study took tomato Ailsa Craig as the test mate-rial.SlMYB48 gene was cloned by RT-PCR,and its bioinformatics,expression and localization were analyzed.The results showed that the open reading frame(ORF)length of tomato SlMYB48 gene was 708 bp,encoding 235 amino acids,and the expression level was the highest in tomato roots,followed by in leaves.The S1MYB48 protein contains a conserved MYB domain,which is an unstable,hydrophilic protein located in the nucleus.SlMYB48 promoter analysis found that it contained a large number of stress response elements.The results of qRT-PCR and RNA-seq database analysis showed that the expression of SlMYB48 gene increased with the extension of treatment time under high salt and biological stress,and decreased under drought stress,suggesting that SlMYB48 gene might be involved in biological and abiotic stress response of tomato.The results of this study provide a reference for further investigation of the function and mechanism of tomato SlMYB48 gene.
NETL
NSTL
万方数据
