Genetic diversity analysis and DNA fingerprinting construction of 61 pro-cessed cucumber germplasm resources based on SCoT markers
In order to find out the genetic diversity of germplasm resources of processed cucumber and provide theoretical basis for the breeding of new varieties.the genetic diversity of 61 processed cucumber germplasm resources was analyzed and DNA fingerprinting was constructed using SCoT technique.The results showed that 8 primers with clear amplifica-tion bands,high polymorphism and good repeatability were selected from 100 SCoT primers.A total of 238 loci of 61 cu-cumber germplasm genomic DNA were amplified by PCR using the selected primers,of which 227 were polymorphic lo-ci,and the average percentage of polymorphic loci was 95.38％.NTSYS-pc 2.1 software was used to calculate the distri-bution of genetic similarity coefficients among the 61 tested materials between 0.588 and 0.920,and the material with the smallest genetic similarity coefficients was 34 and 55,respectively.There were great differences in melon color,leaf shape and fruit shape,indicating that the two were most loosely related.Based on the genetic similarity coefficient,UPG-MA cluster analysis and tree diagram were performed.At the similarity coefficient 0.735,they could be divided into 7 groups.The results indicated that 61 cucumber germplasm material backgrounds were different,but most of the germ-plasm sources were similar,and germplasm from similar habitats were grouped together in the same group.Sixty-one cu-cumber germplasm resources could be identified by DNA fingerprinting of SCoT 12 and SCoT 83,which could provide scientific basis for classification and identification of cucumber.
NETL
NSTL
万方数据
