Comparative analysis of antioxidant active components in dried bitter gourd from different habitats
The main components content and antioxidant activity of 10 groups dried bitter gourd from different habitats were determined,and the dried bitter gourd was analyzed by Fourier transform infrared spectroscopy,which provided the-oretical support for controlling the quality of dried bitter gourd,ensuring its safe use and later development and utiliza-tion.In this experiment,the content of total flavonoids,total phenols and reducing sugars were determined by aluminum nitrate chromogenic method,folin phenol method and dinitrosalicylic acid method,and the antioxidant activity of dried bitter gourd was determined by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical scavenging ability and iron ion reducing ability(FRAP)method.The content of total flavonoids,total phenols and reducing sugar in dried bitter gourd were 1.84,5.24 and 9.00 mg·g-1,respectively.The dried bitter gourd can scavenge DPPH+,and the best IC50 value is 3.42 mg·mL-1.The highest total antioxidant capacity of dried bitter gourd was 192.02 μg·g-1.The main absorption peaks of the infrared spectrum of the dry powder of bitter gourd are all around 3290,2920,1740,1610,1370,1230 and 1020 cm-1.The dried bitter gourd is rich in flavonoids,total phenols and reducing sugars.The combination of one-dimen-sional infrared spectrum and second derivative spectrum can provide scientific data for the identification and analysis of dried bitter gourd,which is helpful for the overall quality control and qualitative analysis of effective components.
万方数据
维普
