Effects of exogenous on the physiological characteristics of tomato seed-lings under Pb stress
In order to study the effect of exogenous GR24 on the growth physiology of tomato seedlings under heavy met-al Pb stress,the tomato variety Shanghai Ruby 908 was used as the experimental material.The experiment was conducted in a plant physiological material culture room,with an average temperature of 28 ℃ and an average relative humidity of 70.85%.Tomato seedlings with consistent growth status and three leaves and one heart were transplanted into 1/2 Hoagland nutrient solution.The experiment included four treatments:CK(complete nutrient solution),GR24(complete nutrient solution+50 nmol·L-1 GR24),Pb(complete nutrient solution+200 mg·L-1Pb),and Pb+GR24(complete nutrient solution+200 mg·L-1+50 nmol·L-1 GR24).The results showed that the Pb stress of 200 mg·L-1 reduced the plant height,root length,and single leaf area of tomato seedlings by 21.03%,35.15%,and 27.27%,respectively.Compared with individual Pb stress treatment,applying exogenous GR24 can significantly increase the content of chlorophyll,carotenoids,soluble sugars,soluble proteins,and bioaccumulation in tomato seedling leaves;significantly increase the activity of antioxidant enzymes such as SOD,POD,CAT,and root activity;reduce the content of H2O2,O2-·,proline and MDA in leaf.There-fore,exogenous application of 50 nmol·L-1 GR24 can alleviate the toxic effect of Pb stress on the growth of tomato seed-lings.