中国瓜菜2024,Vol.37Issue(5) :115-123.DOI:10.16861/j.cnki.zggc.202422.0772

外源独角金内酯对铅胁迫下番茄幼苗生理特性的缓解效应

Effects of exogenous on the physiological characteristics of tomato seed-lings under Pb stress

史沉鱼 韦芳灵 黄彩腰 邓晰朝
中国瓜菜2024,Vol.37Issue(5) :115-123.DOI:10.16861/j.cnki.zggc.202422.0772

外源独角金内酯对铅胁迫下番茄幼苗生理特性的缓解效应

Effects of exogenous on the physiological characteristics of tomato seed-lings under Pb stress

史沉鱼 1韦芳灵 1黄彩腰 1邓晰朝1
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作者信息

  • 1. 广西蚕桑生态学与智能化技术应用重点实验室·广西现代蚕桑丝绸协同创新中心·微生物及植物资源开发利用广西高校重点实验室·河池学院化学与生物工程学院 广西河池 546300
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摘要

为了研究外源独角金内酯(GR24)对重金属铅胁迫下番茄幼苗生长生理的影响,以番茄品种上海红宝石908为材料,在河池学院植物生理材料培养室进行试验,培养室平均温度28 ℃,平均相对湿度为70.85%.选择生长状态较为一致且为3叶1心的番茄幼苗移栽至1/2 Hoagland营养液中,试验共设4个处理,CK(完全营养液)、GR24(完全营养液+50 nmol·L-1 GR24)、Pb(完全营养液+200 mg·L-1 Pb)、Pb+GR24(完全营养液+200 mg·L-1 Pb+50 nmol·L-1GR24).研究了 GR24对正常生长和铅胁迫下番茄幼苗的长势、抗氧化系统和渗透调节物质等的影响.结果表明,与CK相比,200 mg·L-1的Pb胁迫处理番茄幼苗的株高、根长、单叶面积分别降低了 21.03%、35.15%、27.27%.与单独Pb胁迫处理相比,施加外源GR24可以明显提高番茄幼苗叶片中叶绿素、类胡萝卜素、可溶性糖、可溶性蛋白含量(SP)和生物积累量;显著提高POD、CAT等抗氧化酶活性和根系活力;降低植株叶片H2O2、O2-·、脯氨酸和MDA含量.综上,外源施加50 nmol·L-1的GR24能加快铅胁迫下番茄幼苗叶绿素合成速度,提高叶片抗氧化能力,促进番茄苗期生长,缓解铅胁迫对番茄幼苗生长的毒害作用.

Abstract

In order to study the effect of exogenous GR24 on the growth physiology of tomato seedlings under heavy met-al Pb stress,the tomato variety Shanghai Ruby 908 was used as the experimental material.The experiment was conducted in a plant physiological material culture room,with an average temperature of 28 ℃ and an average relative humidity of 70.85%.Tomato seedlings with consistent growth status and three leaves and one heart were transplanted into 1/2 Hoagland nutrient solution.The experiment included four treatments:CK(complete nutrient solution),GR24(complete nutrient solution+50 nmol·L-1 GR24),Pb(complete nutrient solution+200 mg·L-1Pb),and Pb+GR24(complete nutrient solution+200 mg·L-1+50 nmol·L-1 GR24).The results showed that the Pb stress of 200 mg·L-1 reduced the plant height,root length,and single leaf area of tomato seedlings by 21.03%,35.15%,and 27.27%,respectively.Compared with individual Pb stress treatment,applying exogenous GR24 can significantly increase the content of chlorophyll,carotenoids,soluble sugars,soluble proteins,and bioaccumulation in tomato seedling leaves;significantly increase the activity of antioxidant enzymes such as SOD,POD,CAT,and root activity;reduce the content of H2O2,O2-·,proline and MDA in leaf.There-fore,exogenous application of 50 nmol·L-1 GR24 can alleviate the toxic effect of Pb stress on the growth of tomato seed-lings.

关键词

番茄幼苗/铅胁迫/GR24/生理指标/缓解效应

Key words

Tomato seedlings/Pb stress/GR24/Physiological indicators/Alleviative effect

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基金项目

广西自然科学基金(2024GXNSFAA010145)

广西教育厅科研项目(2021KY0623)

河池学院科研项目(2021GCC023)

河池学院科研项目(2021GCC016)

河池学院科研项目(2021GCC017)

河池学院教学改革项目(2022EA002)

桂西北特色植物资源开发与功能研究中心研究平台项目(2017-05-02)

出版年

2024
中国瓜菜
中国农业科学院郑州果树研究所

中国瓜菜

北大核心
影响因子:0.452
ISSN:1673-2871
参考文献量37
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