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外源褪黑素对盐胁迫下萝卜幼苗生长的影响

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土壤盐渍化严重制约萝卜高效生产,褪黑素(melatonin,MT)作为天然抗氧化剂,在响应植物非生物胁迫中发挥重要作用.以满堂红萝卜为试验材料,分别采用浓度为0、50、100、150、200μmol·L-1的MT进行喷施处理,筛选对萝卜生长最佳的MT浓度;采用基质盆栽,以200 mmol·L-1 NaCl浇灌作为盐胁迫环境,以0 μmol·L-1 MT(T1)和筛选出的最佳MT浓度(T2,150 μmol·L1)溶液喷施萝卜幼苗为处理组,非盐胁迫与未喷施MT作对照(CK).结果表明,与CK相比,盐胁迫显著降低萝卜幼苗株高(定植后29 d除外)、茎粗、株幅、叶长、叶宽(定植后29 d除外)等指标,喷施150 μmol·L-1 MT可提高定植25 d后的萝卜幼苗株高、茎粗、株幅、叶长、叶宽和叶绿素含量,增强SOD和CAT活性,降低MDA含量;其中定植后29 d,喷施150 μmol·L-1MT和0 μmol1L-1MT相比,株高、茎粗、叶长、叶宽和叶绿素含量分别提高了 26.79%、28.16%、16.81%、29.11%、7.72%,SOD和CAT活性分别显著提高了 32.12%和40.76%,MDA含量显著降低了 38.92%,表明喷施150 μmol ·L-1外源MT可有效缓解盐胁迫对萝卜幼苗的伤害,有效促进萝卜幼苗生长.
Effects of exogenous melatonin on growth of radish seedlings under salt stress
Soil salinization seriously restricts the efficient production of radish.Melatonin(MT),as a natural antioxidant,plays an important role in responding to plant non-biological stress.Mantanghong radish was used as experimental materi-al to be sprayed by MT with concentrations of 0,50,100,150 and 200 μmol·L-1,respectively,to screen the best MT concentration for radish growth.Then,a matrix pot was used to irrigate radish with 200 mmol·L-1 NaCl as salt stress envi-ronment,spray radish with 0 μmol·L-1 MT(T1)and the optimal MT concentration(T2)solution as treatment group,and control group(CK)was used between non-salt stress and non-spray MT.The results showed that compared with CK,salt stress significantly decreased the plant height(except 29 d after planting),stem diameter,leaf length and leaf width(except 29 d after planting)of radish seedlings.Spraying 150 μmol·L-1 MT could increase the plant height,stem diame-ter,plant width,leaf length,leaf width and chlorophyll content of radish seedlings on 25 days after implantation.The activity of SOD and CAT was increased,and the content of MDA was decreased.On 29 days after implantation,compared with 150 μmol·L-1 MT and 0 μmol·L-1 MT,plant height,stem diameter,leaf length,leaf width and chlorophyll content increased by 26.79%,28.16%,16.81%,29.11%and 7.72%,respectively.SOD and CAT activities were significantly increased by 32.12%and 40.76%,respectively,and MDA content was significantly decreased by 38.92%,indicating that spraying 150 μmol·L-1 exogenous MT could effectively alleviate the damage of salt stress on radish seedlings and pro-mote the growth of radish seedlings.

RadishSalt stressMelatoninSeedling growth

彭笑洁、崔玉莹、王浩、张梓贺、武春成、谢洋

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河北省特色园艺种质挖掘与创新利用重点实验室·河北科技师范学院园艺科技学院 河北秦皇岛 066004

盐胁迫 褪黑素 幼苗生长

河北省现代农业产业技术体系建设专项国家级大学生创新创业训练计划(2022)

HBCT2024140205202210798017

2024

中国瓜菜
中国农业科学院郑州果树研究所

中国瓜菜

北大核心
影响因子:0.452
ISSN:1673-2871
年,卷(期):2024.37(5)
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