Transcriptome analysis of transgenic tomato callus tissues from Wus2 and IPT
AC(Ailsa Craig)tomato(Solanum lycopersicum L.)was used as the experimental material and the Fast-TrACC(fast created Agrobacterium co culture)Agrobacterium transformation system was used in this study.RNA-Seq sequenc-ing and fluorescence quantitative PCR technology were used to compare the gene expression differences between the cal-lus-like tissues formed after transforming DRs(Wus2 and IPT)and the common hypocotyls.The analysis of gene expres-sion results showed that 60 differentially expressed genes were enriched in the hormone signal transduction pathway,in-cluding 34 upregulated genes and 26 downregulated genes.The key genes for somatic embryo formation,ECP63,AGL15,FUS3,ABI3,WUS,and CUC2,are upregulated by more than 4-fold expression;the expression levels of ENOD93 and CKX2 genes in callus-like tissues increased by more than 1000 times.The former encodes the early nodulin protein ENOD93,while the latter encodes cytokinin oxidase 2,which catalyzes the degradation of cytokinin.Low expression of genes involved in nitrogen assimilation and photosynthesis.The research results can lay a foundation for a better under-standing of the molecular mechanism of tomato callus-like tissues formation and the discovery of key regulatory genes,providing a theoretical basis for tomato in plant transformation.
万方数据
维普
