Infection process and physiological and biochemical difference between clubroot-resistant and-susceptible Chinese cabbage lines
Clubroot is a worldwide soil-borne disease of the cruciferous plants caused by Plasmodiophora brassicae,lead-ing to serious economic losses to cabbage and other Brassica crops.In order to clarify the clubroot resistance mechanism of Brassica rapa,this study was conducted to detect the differences of infection process,defense enzyme activity and the content of MDA and soluble protein between clubroot-resistant(DH40R)and clubroot-susceptible(DH199S)Chinese cabbage lines.The results showed that the primary(root hair infection)stage was at 2 day after inoculation(dai)in both clubroot-resistant(DH40R)and clubroot-susceptible(DH199S)Chinese cabbage lines.With the extension of the infec-tion time,cortical infection was blocked and showed complete P.brassicae resistance in DH40R,while in DH199S,swol-len root occurred in the root after 8 dai.The defense enzyme activity and the content of MDA and soluble protein were measured at 2,8 and 13 dai.The activity of SOD in DH40R after inoculation is significantly higher than the mock at the whole experimental stage,the highest peak was reached at 8 dai,which was significantly higher than that of DH199S.The CAT activity of DH40R after inoculation reached the highest peak 1 572.68 μmol·mL-1·min-1 at 8 dai,which was signifi-cantly higher than the mock of DH40R and the inoculated and uninoculated DH199S.The MDA content of DH40R was significantly lower than that of DH199S at 8 and 13 dai.The soluble protein content in DH40R was higher than that of DH199S at 8 and 13 dai.Overall,these results can lay foundation for further exploration of resistance mechanism of B.ra-pa against P.brassicae infection.
Chinese cabbageClubrootInfectionPhysiological and biochemical index
万方数据
维普
