为揭示糙皮侧耳原基发育的潜在调控物质,采用代谢组学的方法对糙皮侧耳菌丝体和原基细胞代谢物进行检测分析.结果表明,菌丝体与原基细胞的代谢物具有显著差异.采用正交偏最小二乘判别分析(OPLS-DA),以VIP(varible importance in the projection)≥1和倍数变化≥2或≤0.5为条件共筛选到400种差异代谢物.调控通路分析表明,这些差异物质涉及33条代谢通路,其中丙氨酸、天冬氨酸和谷氨酸、氨酰tRNA生物合成、嘌呤代谢等8条代谢通路具有极显著影响.谷氨酸和谷氨酰胺涉及p＜0.01的大部分代谢通路,可能在糙皮侧耳原基发育过程中具有重要调控作用.研究结果为糙皮侧耳以及其他大型真菌原基发育机制的探索提供了理论依据.
Abstract
In order to reveal the potential regulatory substances involved in primordium development of Pleurotus ostrea-tus,the metabolomics was used to detected the cellular metabolites in mycelium and primordium.The results showed that there were significant differences between the cellular metabolites in mycelium and primordium.A total of 400 differen-tial metabolites were screened under the condition of VIP(varible importance in the projection)≥1 and fold change≥2 or ≤0.5 using orthogonal partial least squares discriminant analysis(OPLS-DA).According to pathway analysis,these differential substances might involve 33 metabolic pathways,among which 8 metabolic pathways,including alanine,as-partate and glutamate,aminoacyl tRNA biosynthesis and purine metabolism,had extremely significant effects.Glutamate and glutamine involved in most of the metabolic pathways with p＜0.01,therefore they might play an important regulato-ry role in primordium development of P.ostreatus.This study provides a theoretical basis for exploring the development mechanism of P.ostreatus and other macrofungus.
维普
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