Molecular identification,biological characteristics and artificial cultiva-tion of Armillaria strain DJ1
To optimize the artificial culture conditions of Armillaria,using the Armillaria strain DJ1 as the research object,the author conducted molecular identification and biological characteristics observation to select the optimal medium and culture conditions.The results revealed that the phylogenetic tree constructed based on the ITS sequence indicated that the strain DJ1 belongs to Armillaria gallica.The optimal culture conditions for DJ1 growth were PDA medium with sawdust 100 g,bran 5 g,water 1 L,pH=6,and cultivation temperature of 23 ℃.The results of mushroom emergence showed that fruiting bodies could be obtained in PDA+2％/4％/6％/8％cornmeal medium and sawdust medium.Among them,the me-dium with 4％cornmeal had the best mushroom production effect and the fastest mushroom emergence time.With the in-crease of cornmeal addition,the mushroom yield of the fruiting bodies of Armillaria increased first and then decreased.The results of this study can provide a reference for the rapid cultivation and artificial domestication of Armillaria.
ArmillariaEdible and medicinal mushroomsBiological characteristicsArtificial domestication
万方数据
维普
