Simple and rapid screening of organophosphorus and carbamate pesticide residues is of great significance for food safety.In this study,the authors designed and developed a one-step rapid test strip using enzyme inhibition method,with the diazo red RC salt as indicator and indoxyl acetate as substrate of acetylcholinesterase(AChE).The bottom of the test strip is a plastic rubber plate,which is successively pasted with sample pad,enzyme pad,block pad,substrate pad,adsorption pad and nitrocellulose(NC)film that fixed with diazo red RC salt.Qualitative or quantitative analysis of pesti-cide residues can be achieved by detecting changes in the color of the detection area,and the operation method is simple and fast.The detection limits of different pesticides are as follows:0.6 mg·L-1 for malathion,0.4 mg·L-1 for omethoate,1.3 mg·L-1 for acephate,0.1 mg·L-1 for dichlorvos,0.1 mg·L-1 for carbofuran,0.07 mg·L-1 for aldicarb.It shows good reproducibility and high sensitivity in actual fruit and vegetable samples.
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