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黄瓜种质资源果实性状遗传多样性分析

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为深入了解黄瓜果实性状的遗传多样性,优化育种策略,对221份种质资源的13个果实农艺性状进行遗传多样性分析、相关性分析、主成分分析、聚类分析.结果表明,221份黄瓜种质资源的遗传多样性指数平均为1.392,平均变异系数为30.78%.相关性分析表明,除瓜瘤大小外,其他12个性状均有与其关联性较高的性状;主成分分析表明,前4个主成分(PC1~PC4)的累积贡献率为68.82%,包含了黄瓜果实性状的大部分信息.通过表型性状及分子标记分别对种质资源进行聚类分析,结果表明,在欧式距离为0.003时,将221份种质资源的表型性状分为5类,每一类分别包含90、65、9、38、19份种质资源;在欧式距离为1.400时,将221份种质资源采用分子标记分为5类,分别包含了 35、70、45、54、17份种质资源.表型数据和分子标记聚类结果对比,发现两者具有一定的吻合度,但仍存在差异.研究结果为优良育种亲本的选配奠定了一定的基础,也为后续工作提供了参考.
Genetic diversity analysis of fruit-related traits in cucumber germplasm resource
To understand the genetic diversity of cucumber fruit traits and optimize breeding strategies,genetic diversity analysis,correlation analysis,principal component analysis and cluster analysis were performed for 13 fruit agronomic traits of 221 germplasm resources.The results showed that the average genetic diversity index of 221 cucumber germ-plasm resources was 1.392,with the average coefficient of variation of 30.78%.Correlation analysis showed that except the size of fruit tumor,the other 12 traits had high correlation.Principal component analysis showed that the cumulative contribution rate of the four principal components(PC1-PC4)was 68.82%,which contained most of the information of cucumber fruit traits.Cluster analysis was conducted on germplasm resources using phenotypic traits and molecular mark-ers.The results showed that the phenotypic traits of 221 germplasm resources were divided into 5 categories when the Eu-clidean distance was 0.003,and each category contained 90,65,9,38 and 19 germplasm resources,respectively.When the Euclidean distance was 1.400,the molecular markers of 221 germplasm resources were divided into 5 categories,in-cluding 35,70,45,54 and 17 germplasm resources,respectively.The comparison between phenotype data and molecular marker clustering results showed that there was a certain degree of agreement between them.The results have laid a foun-dation for the selection of excellent breeding parents and provided a reference for the subsequent work.

CucumberGenetic diversityPhenotypic charactersMolecular marker

王荣、董邵云、刘小萍、官健涛、苗晗、李森、张圣平

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山西农业大学园艺学院 山西太谷 030801

蔬菜生物育种全国重点实验室·中国农业科学院蔬菜花卉研究所 北京 100081

黄瓜 遗传多样性 表型性状 分子标记

2024

中国瓜菜
中国农业科学院郑州果树研究所

中国瓜菜

北大核心
影响因子:0.452
ISSN:1673-2871
年,卷(期):2024.37(7)