Bioinformatics and expression analysis of DoCDPK1 gene under low tem-perature stress during tuber expansion stage of yam
Calcium-dependent protein kinase plays an important role in plant growth and osmotic stress.Using Dahechangyu and Bikeqi yam as experimental materials,the calcium dependent protein kinase gene DoCDPK1 was cloned and bioinformatic analysis was performed to lay a foundation for the functional study of DoCDPK1.The structure of DoCDPK1 was analyzed by bioinformatics and subcellular localization of DoCDPK1 protein was performed by con-structing transient expression vector.qRT-PCR was used to analyze the expression patterns of DoCDPK1 at different developmental stages and under low temperature stress in different yam varieties.The results indicated:(1)The length of DoCDPK1 gene sequence was 2023 bp,encoding 521 amino acids.(2)The consistency of the protein sequence between DoCDPK1 and CDPK protein sequence of Dioscorea cayenensis subsp.was 97％,and DoCDPK1 had STKc_CAMK domain.(3)Transient expression analysis showed that DoCDPK1 protein was localized in cell nucleus and membrane.(4)The CDPK activity in Dahechangyu was higher,and DoCDPK1 might be involved in regulating the growth and devel-opment of yam tuber at the later stage of enlargement.(5)After low temperature stress at 4 ℃,CDPK activity decreased,and the expression of DoCDPK1 was up-regulated,with differences in expression levels observed under different time treatments.In conclusion,DoCDPK1 gene may be involved in regulating the growth and development of yam tuber and its response to low temperature stress.
YamCalcium-dependent protein kinaseSubcellular localizationLow temperature stressGene expression
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