Cloning and expression analysis of StWRKY51 gene in potato under heat stress
To explore the response characteristics of potato StWRKY51 gene under high temperature stress,the StWRKY51 gene was cloned from leaf of the potato cultivar Russet Burbank by RT-PCR technology,and bioinformatics analysis of this gene and the real-time fluorescence quantitative PCR for StWRKY51 expression in potato under the high temperature stress were performed in this study.The results were as follows:The open reading frame of StWRKY51 gene is 696 bp,en-coded 231 amino acids;the amino acid sequence of StWRKY51 protein was more than 80％similar to that of Solanum pennellii,Solanum lycopersicum and Capsicum annuum;the StWRKY51 was belong to an acidic and stable hydrophilic protein,and the ratio of irregular curl in the secondary structure of protein was up to 63.64％.The domain analysis showed that StWRKY51 gene encoding protein had a typical WRKY conserved domain,and the zinc finger structure is C2H2 type,belonging to the Group Ⅱ subfamily.The qPCR results showed that the expression of StWRKY51 gene was significantly induced under high temperature treatment,indicating that StWRKY51 gene may play a regulation role in pota-to resistance to high temperature stress.The research provided a reference for further study on the function of StWRKY51 response to high temperature stress in potato.
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