Observation on meiosis of pollen mother cells of super-male plants in As-paragus officinalis L.
Meiosis is a key process in sexual reproduction.In order to clarify the specific situation and the effect of abnor-mal meiotic behavior of pollen mother cells(PMCs)on the growth and development of gametes in super-male asparagus plants,the improved phenol fuchsin method was used to systematically observe the meiosis process of 986 PMCs of 58 su-per-male asparagus plants.The results showed that:(1)The PMCs of super-male plants carried meiosis and cytokinesis si-multaneously,with a chromosome configuration of 10 bivalents and the tetrad of a cross shaped structure.(2)At meta-phase Ⅰ,the proportion of small rod bivalents was 96.55％,tetravalent was 8.62％,and chromosome sticking was 22.41％.(3)At anaphase Ⅰ,the proportion of lagging chromosomes was 12.07％,chromosome bridges was 13.79％,and the propor-tion of micronucleus was 6.59％in the division phase.(4)At metaphase Ⅱ to telophase Ⅱ,the proportion of asynchroniza-tion of chromosomes segregation was 34.45％.In the whole process of meiosis,the prophase Ⅰ is the longest.There was paired as 10 bivalents at meiosis metaphase Ⅰ,thus it is a diploid species.The abnormal behavior of meiosis mainly oc-curs in metaphase Ⅰ and anaphase Ⅰ.Based on the observation of the meiosis process of PMCs of the super-male,it can not only enrich the cytogenetic content of asparagus,but also provide the necessary cytogenetic information for asparagus of developmental biology,cross breeding,gene localization,and so on.
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