Cloning and expression analysis of tomato SlDOGL4 transcription factor gene
In order to clarify the salt-tolerance effect of SlDOGL4 gene in tomato,the cultivated tomato Ailsa Craig was used as the experimental material to clone this gene and analyze its physicochemical properties and expression characteris-tics through bioinformatics methods.The results showed that the full length of CDS of SlDOGL4 gene was 660 bp,encod-ing 219 amino acids.Conserved domain analysis showed that the gene contained only one DOG1 domain,which belonged to the DOG1 family of bzip transcription factor.Phylogenetic relationship showed that SlDOGL4 was closely related to StDOGL4 protein in potato.The results of tissue expression profile showed that SlDOGL4 gene was mainly expressed in root and fruit at the broken color stage.Subcellular localization results showed that SlDOGL4 protein was located in the nu-cleus.SlDOGL4 gene promoter has a variety of cis-elements related to abiotic stress and hormone response.The ProSlDOGL4::GUS fusion expression vector was constructed to genetically transform tomato to detect promoter activity.GUS histochem-ical staining showed that SlDOGL4 was expressed in roots,stems,growing points and seeds,indicating that SlDOGL4 played an important role in the whole growth and development process of tomato.Analysis of transcriptome data from pub-lic databases showed that the expression of SlDOGL4 gene was induced by salt,drought,cold and heat stress to varying de-grees.In order to further verify the function of SlDOGL4 gene,the transcription level of SlDOGL4 gene showed a trend of first increasing and then decreasing,and reached the highest transcription level after 2 hours of salt treatment.These results laid a foundation for exploring the function of SlDOGL4 gene in tomato salt tolerance.
万方数据
维普
